Cryo-EM structure of human LRRC8A
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 7xzh
Q-score: 0.322
To Be Published
- Volume-regulated anion channel subunit lrrc8a (94 kDa, Protein from Homo sapiens)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Volume regulated anion channel (Complex from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Cholesterol (386 Da, Ligand)
LRRC8A:C conformation 2 (oblong) top mask
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8dr8
Q-score: 0.463
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
LRRC8A:C conformation 2 (oblong) LRR mask
Resolution: 3.98 Å
EM Method: Single-particle
Fitted PDBs: 8dra
Q-score: 0.281
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
LRRC8A:C conformation 2 (oblong)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8dre
Q-score: 0.382
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
LRRC8A:C conformation 1 (round) top focus
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8drk
Q-score: 0.47
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
LRRC8A:C conformation 1 (round) LRR focus 1
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8drn
Q-score: 0.258
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
LRRC8A:C conformation 1 (round)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8ds3
Q-score: 0.327
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
LRRC8A:C in MSPE3D1 nanodisc top focus
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8ds9
Q-score: 0.39
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Lrrc8a:c channel (620 kDa, Complex from Mus musculus)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8dsa
Q-score: 0.326
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Lrrc8a:c channel (620 kDa, Complex from Mus musculus)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)
LRRC8A(T48D):C conformation 2 top focus
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8f74
Q-score: 0.457
Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG
Nat Struct Mol Biol (2023) 30 pp. 841-852 [ DOI: doi:10.1038/s41594-023-00944-6 Pubmed: 36928458 ]
- Volume-regulated anion channel subunit lrrc8a,soluble cytochrome b562 (105 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Lrrc8a:c (620 kDa, Complex from Mus musculus)
- Volume-regulated anion channel subunit lrrc8c (93 kDa, Protein from Mus musculus)